Expression pattern of hoxd3 gene during early development of wild-type zebrafish embryos / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To investigate the expression pattern of hoxd3 gene during early embryogenesis and angiogenesis of wild-type zebrafish.</p><p><b>METHODS</b>Total RNA was extracted from embryos of zebrafish in different development stages by trizol. The cDNA of hoxd3 gene was amplified by RT-PCR. The RT-PCR product was ligated to pCS(2+) vector by T4 DNA ligatase polymerase and sequenced. T3 RNA polymerase in vitro transcription system was used to obtain the probe of digoxin-labeled anti-sense mRNA of hoxd3 gene. The expression pattern of hoxd3 was detected by whole embryo in situ hybridization (WISH) with anti-sense mRNA probe.</p><p><b>RESULTS</b>pCS(2+)-hoxd3 plasmid was successfully constructed, which was used to prepare anti-sense mRNA probe of hoxd3 in vitro. Expression pattern of hoxd3 gene was detected by WISH during zebrafish early embryogenesis and angiogenesis. It was observed that hoxd3 mRNA was expressed at the junction region of midbrain and hindbrain in wild-type zebrafish in embryos at 24 ≊72h postfertilization(hpf).</p><p><b>CONCLUSION</b>hoxd3 gene is mainly expressed in nervous system of wide-type zebrafish embryos.</p>

Expression of pax5 gene in wildtype zebrafish embryos / 第二军医大学学报

Objective To study the role and related mechanisms of pax5 gene during the development of the embryos, especially in the early development of B cell and midbrain, and to observe the expression of pax5 gene in wild type zebra fish embryos. Methods Total RNA of Tue Un gen wild type zebra fish embryos was extracted to obtain cDNA of pax5 gene by RT-PCR with specific primers. The c DNA of pax5 gene and pCS2+ vector were double digested with EcoR I and Xba I, and then ligated by T4 DNA ligase. The recombinant vector was verified by double digestion, colony PCR screening and sequencing. The verified recombinant vector was then used to synthesize digoxin labeled anti-sense mRNA probe of pax5 gene using T3 RNA polymerase in vitro transcription system. The generated probes were used to detect pax5 gene expression in zebra fish embryos by whole-mount in situ hybridization. Results The pCS2+-pax5 recombinant plasmid and the probe of digoxin-labeled anti-sense mRNA of pax5 gene was successfully constructed. Whole-mount in situ hybridization showed that pax5 gene was expressed in the cerebellum and midbrain hindbrain boundary from 18-72 h post-fertilization (hpf). In the cochlea pax5 gene was expressed from 24-72 hpf and the expression was increased as time went by. From 18-48 hpf pax5 gene is found in the notochord. Conclusion Pax5 gene is highly expressed in the brain and notochord of zebra fish embryos. Pax5 gene expression is found in the cochlea of the Tue bingen wild type zebra fish' s embryos for the first time. It is suggested that pax5 may play an important role in the development of the nervous system in the early zebra fish embryos.